1 Bone meta (-)Bone meta (+) n=101n=57p-value Age (min-max)73.0 (51-88)71.0 (55-86)0.027 BMI (min-max) PrePSA (min-max) 24.0 (16.0-32) 20.0 (2.0-982.0) 23.0 (17-28) 139.0 (7.8-6262.0) 0.037 0.001 Gleason score0.006 ≤6≤624 (23.8)3 (5.3) 729 (28.7)15 (26.3) ≥8≥848 (47.5)39 (68.4) Posttreatment PSA nadir 0.04 (0.01-30.4)0.2 (0.01-79.8)0.04 A. C. B. T1 and T2 T3 and T4 Lee et al. Supp Fig 1

2 LNCaP22RV1 Lee et al. Supp Fig 2

3 Lee et al. Supp Fig 3 A. * * * * * * * B. C. *

4 BMPRII ActRIIa ActRIIb β-actin Con siRNA BMPRII siRNAActRIIa siRNAActRIIb siRNA BMPRIa BMPRIb ActRIa β-actin Con siRNABMPRIa siRNABMPRIb siRNA ActRIa siRNA Smad1 Smad5 Smad8 β-actin Con siRNASmad1 siRNASmad5 siRNA Smad8 siRNA Lee et al. Supp Fig 4

5 Wnt5a β-actin LNCaP22RV1 WPMY-1 HS-5 WPMY-1 H-5 Stromal Cell line - - - - Lee et al. Supp Fig 5

6 Lee et al. Supp Fig 6 22RV1 SP600125:10uM, Calphostin C:100nM Wnt5a-+++ Inhibitor-- SP600125 Calphostin C JNKiPKCi Wnt5a-+++ Inhibitor-- SP600125 Calphostin C JNKiPKCi BMP-6  -actin

7 Lux ATG TATACAT NF-  B Lee et al. Supp Fig 7

8 PCK NF-  B receptor Lee et al. Supp Fig 8