1 Fisiología microbiana Bota 6006flagelo tallo

2 1 ~1500 nucleotides Secondary structure of 16S rRNA

3 Evolutionary distance - # de nucleótidos diferentes y se usan para calcular distancia.Maximum parsimony - # de nucleótidos diferentes, sus posiciones y la naturaleza de las diferencias.

4 EL ARBOL DE LA VIDA basado en secuencias de genes de RNA ribosomal 16S la raíz del árbol de la vida representa una forma ancestral común Tres linajes distintivos (2 Prokarióticos/1 Eucariótico) Archaea, Bacteria & Eucaria Archaea & Eucaria son evolutivamente más relacionados Archaea están evolutivamente más cercanas a la forma ancestral (más primitivos). Eucariotas están más distanciados de la forma ancestral (más evolucionados) origen termofílico de los seres vivos

5 Yes No 70S 80S Met Caracteristicas Bacteria Archaea EukaryaPeptidoglycan Yes No Lipids Ester linked Ether linked Ribosomes 70S 80S Initiator tRNA formyl-Met Met Introns in tRNA Ribosomes sensitive to diphteria toxins RNA polymerase 1 (4 subunits) Several (8-12 subunits) 3 (12-14 subunits) Histones

6 Prokaryotic cell wall The primary function of the CW is to protect the cell from bursting. There are only two types of CW in Bacteria:

7 Peptidoglycan Chemical structure

8 G+ Cell Wall Covalently linked To the peptidoglycan 15-30 nm thick

9 Teichoic acid attachmentPolyanionic polymers of ribitol-PO4 Or glycerol-PO4. Attachment to peptidoglycan N-acetil ácido murámico (mureína) or glycerol

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11 LPS It produce a permeability barrier to hydrophobic compounds:antibiotics, dyes (eosin-methylene blue), bile salts Lipido A Endotoxina, fiebre, necrotización de tejidos fallo cardíaco Polisacárido O-específico desata reacciones alérgicas Polisacárido medular

12 Porin are major proteins in the outer envelop that form small non-specifichydrophilic channels that allow the diffusion of low molecular weight neutral or charged solutes. Examples are LamB, OmpF, OmpC, Tsx.

13 Periplasm It is a aqueous compartment containing protein, salts,oligosaccharide, and peptidoglycan. What happens in this space? Specialized activities such as 1) oxidation-reduction reactions 2) osmotic regulation 3) solute transport 4) protein secretion 5) degradation by phosphatases and nucleases. 6) Detoxification

14 Cell membrane >100 different Proteins. Integral and peripheral.Responsible for solute transport, electron transport, photosynthetic electron transport, the establishment of electrochemical gradients, ATP synthesis, biosynthesis of lipids, biosynthesis of cell wall polymers, secretions of proteins, the secretion and uptake of intercellular signals, and responses to environmental signals.

15 Ácidos grasos Rol estructural en la membrana citoplasmica

16 Archaea

17 FtsZ is a cell division protein related to tubulin.Cytoskeleton Phase contrast Nucleoid stain FtsZ stain Nucleoid stain FtsZ stain FtsZ is a cell division protein related to tubulin.

18 Streptococcus hemolyticusWall band

19 Cell Shape Rod-shape

20 Bacterial Flagella perítrico lofótrico monotrico amfitrico Los flagelos son filamentos helicoidales y rígidos de un diámetro de ~20nm y que rota como una hélice. Estos consisten de: basal body, a hook, filament, motor, switch, export apparatus, capping and junction proteins.

21 Flagella and its componentsCentral rod FlgB,C,F FlgH protein FlgI protein Basal Body MS ring FliF protein C ring

22 The motor H+ pass across the CM thru the MotA,Bwhich provide the torque to rotate the rotor. C ring (FliM, N) Rotor (FliG proteins) Startor (MotA, B proteins)

23 The motor: how does it change direction?The direction of the rotation results in the direction of the cell (North, south…) and this is related to CheY-P which are chemotactic proteins and binds FliM. C ring (FliM, N) Rotor (FliG proteins) Startor (MotA, B proteins)

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26 Chemiotactic effect on motility

27 The motor FliC is not identical in all bacteria.20 to 65 kD more importantly only the C and N-terminal seem to be conserved (~60%). The filament grows from the hook To the capping protein by adding flagellin monomers as it needs. Furthermore, a cell could have more than one type of flagellin. E. coli (1), Caulobacter sp. (3).

28 Summary of steps in Flagella biosynthesisGram-negative Bacteria