1 Supplementary Figure S1. Methods of the study.Isolation of Trypanosoma cruzi stocks from humans, vectors and didelphids from Amazonas State, Brazil DTU-level genotyped stocks Inoculation of 20 male Swiss mice with each DTU (9 TcI – 180 mice; 10 TcIV – 200 mice) Inoculation of 7 male Swiss mice with each DTU (5 TcI – 35 mice; 2 TcIV – 14 mice) 3º d.a.i. Starting fresh blood examination Starting fresh blood examination Day of maximum mortality 5º d.a.i. 10 mice treated with benznidazole 10 non-treated mice Finishing treatment 24º d.a.i. Mortalitiy parameters 55º d.a.i. Hemoculture and PCR Hemoculture and PCR Finishing fresh blood examination Hemoculture Euthanasia for histopathological analysis % MOR in the acute phase (0-90º d.a.i.) 90º d.a.i. 115º and 205º d.a.i. ELISA ELISA Timeline %MOR in the chronic phase (91-180º d.a.i.) PPP, PP, MDP, Pmax, DPmax, %INF, %FBE, %HC, %PCR, %ELISA at the early chronic, %ELISA at the late chronic phase Susceptibility to beznidazole Frequency of mice with inflammatory process and tissue parasitism