1 Table S3. Primer sequence used for the cloning analysis.KRAS mutation Sequence (5’ ➝ 3’) F-Primer-1* GAATGGTCCTGCACCAGTAA R-Primer-1* GTGTGACATGTTCTAATATAGTCA F-Primer-2** GGATCTTCCAGAGATATCGAATGGTCCTGCACCAGTAA R-Primer-2** CTGCCGTTCGACGATATCGTGTGACATGTTCTAATATAGT BRAF mutation Sequence (5’ ➝ 3’) F-Primer-1* TGCTTGCTCTGATAGGAAAATG R-Primer-1* AGCATCTCAGGGCCAAAAAT PIK3CAex9 mutation Sequence (5’ ➝ 3’) F-Primer-1* CTGTGAATCCAGAGGGGAAA R-Primer-1* ACATGCTGAGATCAGCCAAAT F-Primer-2** GGATCTTCCAGAGATATCCTGTGAATCCAGAGGGGAAA R-Primer-2** CTGCCGTTCGACGATATCACATGCTGAGATCAGCCAAAT *Primer 1 was used for preparing PCR product with Gotaq® DNA polymerase. **Primer 2 was used for preparing PCR product with PrimeSTAR® GXL DNA polymerase. The sequence of vector specific part is on underline.