1 The core facility platform at the Biomedical CentreSandra jovic

2 The platform overview Located at BMCLargest bioscience centre in Scandinavia 1300 researchers Wide range of techniques Open to internal and external users

3 The platform overview Immunohistochemistry BiaCore Bio-PlexRT-PCR (up-and-coming) Sample preparation for: Scanning electron microscopy (SEM) Transmission electron microscopy (TEM) Delphi microscope IVIS Me (Sandra Jovic) Maria Baumgarten Artur Schmidtchen

4 Workflow Immunohistochemistry Tissue sampleProcessing (dehydrate/embed) Sectioning Antigen retrieval Immunohistochemistry

5 Instruments Microtome Leica Microtome RM2255Sectioning of paraffin-embedded specimens 4 µm

6 Instruments PT Link Dako PT LinkReducing the number of operations needed in the pre-treatment process Delayed start

7 Instruments Autostainer Dako Autostainer 48 Up to 48 slides per runTracking all slides Slides treated in the same way

8 Instruments BiaCore BiaCore X100 Study molecular interactions SPRChanges in the refractive index are detected and recorded as RU Terminology (ligand/analyte)

9 Instruments BiaCore Covalent immobilizationHigh affinity capture CM-chips (carboxymethylated dextran) SA-chips (treptavidin) NTA-chips (nitrilotriacetic)

10 Instruments Bio-Plex Bio-Plex 200Investigation of up to 50 analytes in a small sample volume ELISA/flow cytometery Cell culture supernatants, plasma, serum, lavage, sputum Discounts

11 Instruments Real-Time PCR QuantStudio 7 Flex Real-Time PCR SystemAmplification/detection of nucleic acids Gene expression, miRNA profiling, SNP genotyping, pathogen detection

12 Instruments IVIS IVIS Spectrum instrumentMonitoring of distribution of bio-luminescent or florescent biomarkers Real-time Smaller animals

13 Contact Info Sandra Jovic, Ph.D Division of Infection medicine, Department of Clinical Sciences, Lund University

14 Methods/Techniques Sample prepartion for TEM/SEMTransmission Electron Microscope (TEM) Negative staining Gold labelling Scanning Electron Microscope (SEM) Metal coating Delphi microscope

15 Instruments Delphi DELMIC and Phenom-WorldCombined fluorescence and electron microscopy Detailed structural information with nanoscale resolution

16 Instruments Critical point dryer Baltec CPD 03Dries biological specimens Exchanges water/alcohol with liquid carbon dioxide

17 Instruments Ultramicrotome Reichert UltraCut S ultramicrotome0 to 2,5 microns 60 nm High quality ultra-thin sections for electron microscopy and light microscopy

18 Instruments Fluorescence microscopeOlympus IX73 and BX43 fluorescence microscope Coupled to the ultramicrotome Used for preparation of various specimens for electron microscopical analyses

19 Instruments Automatic contrasting instrumentLeica EM AC20 Contrasting with heavy metal

20 Instruments Sputter coater Leica ACE 200Producing homogenous and conductive metal or carbon coatings for SEM and TEM analysis

21 Contact Info Maria Baumgarten, Biomedical scientist Division of Infection medicine, Department of Clinical Sciences, Lund University

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